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Medical Genetics and Genomics
Q1: Produce a bar chart to show the amounts of DNA produced by students usingboth the silica resin spin column method and the inorganic method. NOTE: Data forthis bar chart can be found in the ‘Coursework 2’ folder of the Assessmentarea of the Blackboard site. Submit a copy of your chart (you may upload it as aJPEG, Tiff or Picture file).Q2: Write one, clear, concise null hypothesis regarding the two different methodsused in this module to extract and purify DNA (50 words maximum).Q3: According to the class data issued on Blackboard, which method gives, onaverage, the highest yield of DNA?Q4: According to the class data issued on Blackboard, which method gives, onaverage, the purest DNA?Q5: According to the class data issued on Blackboard, which method gives the mostconsistent yield of DNA?Q6: According to the class data issued on Blackboard, which method is the leastconsistent with respect to the purity of DNA produced?Q7: What is the role of thymolphthalein in the resin spin column method of DNApurification? Please select the most correct answer.Q8: What is the role of EDTA in the inorganic DNA purification method? Pleaseselect the most correct answer.Q9: When performing QPCR to study differential gene expression, what is the role of‘housekeeping’ genes? Please select the most correct answer.Q10: When performing QPCR to study differential gene expression, what is the roleof intron spanning primers?